Presence of Brachyspira aalborgi and B. pilosicoli in Feces of Patients with Diarrhea
نویسندگان
چکیده
منابع مشابه
Presence of Brachyspira aalborgi and B. pilosicoli in feces of patients with diarrhea.
Anaerobic intestinal spirochetes of the genus Brachyspira are important enteric pathogens in animals, but they have not been extensively studied in humans. The presence of a layer of spirochetes attached by one cell end to the colorectal epithelium is known as intestinal spirochetosis (IS) (4). The clinical significance of this colonization is uncertain, but it has been linked to chronic diarrh...
متن کاملPCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces.
Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces for B. aalborgi and B. pilosicoli respectively were 2x10(2) and 7x10(3) cells p...
متن کاملColonization and risk factors for Brachyspira aalborgi and Brachyspira pilosicoli in humans and dogs on tea estates in Assam, India.
The prevalence of colonization with the anaerobic intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli was investigated in humans (n = 316) and dogs (n = 101) living on three tea estates in Assam, India. Colonization was detected using PCR on DNA from faeces. Nineteen (6%) human faecal samples contained B. aalborgi DNA, 80 (25.3%) contained B. pilosicoli DNA, and 10 (3.2%) co...
متن کاملComparative prevalences of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli as etiologic agents of histologically identified intestinal spirochetosis in Australia.
DNA from gastrointestinal biopsy specimens from 28 Australian patients with histologic evidence of intestinal spirochetosis (IS) was subjected to PCRs to amplify segments of the 16S rRNA and NADH oxidase genes of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli. B. aalborgi was identified in specimens from 24 (85.7%) patients and B. pilosicoli in those from 4 (14.3%) patients (2 of w...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2003
ISSN: 0095-1137
DOI: 10.1128/jcm.41.9.4492.2003